@article {4129,
	title = {Assembly for semi-correlation analysis of samples using cryogenic scanning electron microscopy and cryogenic Raman micro-spectroscopy},
	journal = {MEASUREMENT SCIENCE AND TECHNOLOGY},
	volume = {36},
	year = {2025},
	month = {MAY 31},
	pages = {055903},
	keywords = {BF, MF},
	issn = {0957-0233},
	doi = {10.1088/1361-6501/adc94a},
	author = {Laznicka, Tomas and Kizovsk{\'y}, Martin and Hrubanov{\'a}, Kamila and Je{\v z}ek, Jan and Mrazova, Katerina and Obru{\v c}a, Stanislav and Samek, Ota and Krzy{\v z}{\'a}nek, Vladislav}
}
@article {4196,
	title = {The characterization of halophilic polyhydroxyalkanoate-producing bacteria from brine in Solivar near Pre{\v s}ov (Slovakia)},
	journal = {World Journal of Microbiology and Biotechnology},
	volume = {41},
	year = {2025},
	pages = {505},
	abstract = {<p>The present study aims to isolate and investigate temporal variability of the halophilic and halotolerant microbiota present in brine from former salt mine Solivar, Pre{\v s}ov (Slovakia) especially with respect to with their ability to produce polyhydroxyalkanoates (PHA). Brine sampling was performed in the year 2020 and 2021 and samples were inoculated on the R2A medium with 5\% NaCl for the bacterial isolation. We obtained a total of 53 halophilic isolates and one halotolerant isolate, all of which were tested for their ability to produce PHA via Nile Blue A staining, Raman spectroscopy and Gas chromatography. The low diverse halophilic microbiota was dominated by Proteobacteria members (mainly Halomonas, Halovibrio, and Chromohalobacter sp.) and some of these bacteria represent newly identified taxa. Around 80\% of the isolates were able to produce PHA during growth on glucose-rich media, which highlights the importance of PHA for adaptation to high-salinity environments. Poly(3-hydroxybutyrate) (PHB) was the main type of PHA produced with the yield up to 2.76\&nbsp;g/L in Halovibrio sp. HP20-59. Overall, our investigation pointed out that brine from Solivar shows genetically variable community of halophilic bacteria most of which are capable of accumulation of PHA, hereby confirming the high biotechnological potential of halophilic bacteria.</p>},
	keywords = {BF, MF},
	issn = {1573-0972},
	doi = {10.1007/s11274-025-04737-5},
	url = {https://doi.org/10.1007/s11274-025-04737-5},
	author = {Adhvaryu, Shivani and Kiskova, Jana and Piknova, Maria and Malinicova, Lenka and Beck, Terezia and Buchtikova, Iva and Kourilova, Xenie and Kizovsk{\'y}, Martin and Samek, Ota and Obru{\v c}a, Stanislav and Pristas, Peter}
}
@article { SedlacekAMB19,
	title = {What keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments},
	journal = {Appl. Microbiol. Biotech.},
	volume = {103},
	number = {4},
	year = {2019},
	pages = {1905-1917},
	keywords = {BF, MF},
	doi = {10.1007/s00253-018-09584-z},
	author = {P. Sedl{\'a}{\v c}ek and Slaninov{\'a}, E. and Enev, V. and Koller, M. and J. Nebes{\'a}{\v r}ov{\'a} and I. M{\'a}rov{\'a} and K. Hrubanov{\'a} and V. Krzy{\v z}{\'a}nek and O. Samek and S. Obru{\v c}a}
}
@article {HrubanovaMM17,
	title = {Morphological and Production Changes in Planktonic and Biofilm Cells Monitored Using SEM and Raman Spectroscopy},
	journal = {Microscopy and Microanalysis},
	volume = {23},
	year = {2017},
	pages = {S1},
	keywords = {BF, MF},
	doi = {10.1017/S1431927617006456},
	author = {K. Hrubanov{\'a} and V. Krzy{\v z}{\'a}nek and O. Samek and R. Skoup{\'y} and M {\v S}iler and J Je{\v z}ek and S. Obru{\v c}a and P. Zem{\'a}nek}
}
@article {ObrucaNB17,
	title = {The presence of PHB granules in cytoplasm protects non-halophilic bacterial cells against the harmful impact of hypertonic environments},
	journal = {New Biotechnol.},
	volume = {39},
	year = {2017},
	pages = {10},
	keywords = {BF, MF},
	doi = {10.1016/j.nbt.2017.07.008},
	author = {S. Obru{\v c}a and P. Sedl{\'a}{\v c}ek and F. Mravec and V. Krzy{\v z}{\'a}nek and J. Nebes{\'a}{\v r}ov{\'a} and O. Samek and D. Ku{\v c}era and Pavla Bene{\v s}ov{\'a} and K. Hrubanov{\'a} and M. Milerov{\'a} and I. M{\'a}rov{\'a}}
}
@article { MravecFEMSML16,
	title = {Accumulation of PHA granules in Cupriavidus necator as seen by confocal fluorescence microscopy},
	journal = {FEMS Microbiology Letters},
	volume = {363},
	year = {2016},
	pages = {10},
	keywords = {MF},
	doi = {10.1093/femsle/fnw094},
	author = {F. Mravec and S. Obru{\v c}a and V. Krzy{\v z}{\'a}nek and P. Sedl{\'a}{\v c}ek and K. Hrubanov{\'a} and O. Samek and D. Ku{\v c}era and Pavla Bene{\v s}ov{\'a} and J. Nebes{\'a}{\v r}ov{\'a}}
}
@article { ObrucaPLOSONE16,
	title = {Accumulation of Poly(3-hydroxybutyrate) Helps Bacterial Cells to Survive Freezing},
	journal = {PLoS ONE},
	volume = {11},
	year = {2016},
	pages = {e0157778},
	keywords = {MF},
	doi = {10.1371/journal.pone.0157778},
	author = {S. Obru{\v c}a and P. Sedl{\'a}{\v c}ek and V. Krzy{\v z}{\'a}nek and F. Mravec and K. Hrubanov{\'a} and O. Samek and D. Ku{\v c}era and Pavla Bene{\v s}ov{\'a} and I. M{\'a}rov{\'a}}
}
@article { ObrucaAMB16,
	title = {Evaluation of 3-hydroxybutyrate as an enzyme-protective agent against heating and oxidative damage and its potential role in stress response of poly(3-hydroxybutyrate) accumulating cells},
	journal = {Appl Microbiol Biotechnol},
	volume = {100},
	year = {2016},
	pages = {1365-1376},
	keywords = {MF},
	doi = {10.1007/s00253-015-7162-4},
	author = {S. Obru{\v c}a and P. Sedl{\'a}{\v c}ek and F. Mravec and O. Samek and I. M{\'a}rov{\'a}}
}
@article {SamekSENS16,
	title = {Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16},
	journal = {Sensors},
	volume = {16},
	year = {2016},
	pages = {1808},
	keywords = {MF},
	doi = {10.3390/s16111808},
	author = {O. Samek and S. Obru{\v c}a and M {\v S}iler and P. Sedl{\'a}{\v c}ek and Pavla Bene{\v s}ov{\'a} and D. Ku{\v c}era and I. M{\'a}rov{\'a} and J Je{\v z}ek and Silva Bernatov{\'a} and P. Zem{\'a}nek}
}
@article { KrzyzanekMM15,
	title = {Cryo-SEM and Raman Spectroscopy Study of the Involvement of Polyhydroxyalkanoates in Stress Response of Bacteria},
	journal = {Microscopy and Microanalysis},
	volume = {21},
	number = {S3},
	year = {2015},
	pages = {183-184},
	keywords = {MF},
	doi = {10.1017/S1431927615001713},
	author = {V. Krzy{\v z}{\'a}nek and K. Hrubanov{\'a} and O. Samek and S. Obru{\v c}a and I. M{\'a}rov{\'a} and Silva Bernatov{\'a} and M {\v S}iler and P. Zem{\'a}nek}
}
