02201nas a2200277   4500008004100000022001400041245013800055210006900193300001100262490000700273520133600280653000701616653000701623100002601630700001801656700002201674700002701696700001601723700001701739700002001756700001501776700002101791700002101812700001801833856007201851       2024                        eng d  a2405-844000aRaman microspectroscopy and laser-induced breakdown spectroscopy for the analysis of polyethylene microplastics in human soft tissues0 aRaman microspectroscopy and laserinduced breakdown spectroscopy   ae378440 v103 a<p>People are exposed to microplastics (MPs) on a large scale in everyday life. However, it is not clear whether MPs can also be distributed and retained in certain tissues. Therefore, the development of analytical methods capable of detecting MPs in specific human organs/tissues is of utmost importance. In this study, the use and combination of spectroscopic techniques, namely Raman microspectroscopy and laser-induced breakdown spectroscopy (LIBS), was tested for the detection of polyethylene (PE) MPs in human tonsils. Preliminary results showed that Raman microspectroscopy was able to detect MPs down to 1&nbsp;μm in size and LIBS down to 10&nbsp;μm. In the next step, human tonsils were spiked with PE MPs, and digested. The filtered particles were analyzed using Raman microspectroscopy and LIBS, and complemented by X-ray fluorescence (XRF). The results showed that Raman microspectroscopy could reliably detect PE MPs in spiked human tonsils, while LIBS and XRF served as a reference analytical method to characterize particles that could not be classified by Raman microspectroscopy for their non-organic origin. The results of this study, supported by a current feasibility study conducted on clinical samples, demonstrated the reliability and feasibility of this approach for monitoring MPs in biotic samples.</p>10aBF10aMF1 aParobková, Viktória1 aHolub, Daniel1 aKizovský, Martin1 aKalčíková, Gabriela1 aRozman, Ula1 aUrík, Milan1 aNovotný, Karel1 aSamek, Ota1 aZikmund, Tomáš1 aPořízka, Pavel1 aKaiser, Jozef  uhttps://www.sciencedirect.com/science/article/pii/S2405844024138753