00451nas a2200121   4500008004100000022001400041245013400055210006900189260001100258300001100269490000700280856004200287       2022                        eng d  a2235-298800aRaman Spectroscopy-A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings0 aRaman SpectroscopyA Novel Method for Identification and Characte  cAPR 22  a8664630 v12  uhttp://drupal.isibrno.cz/en/node/357501261nas a2200109   4500008004100000245012300041210006900164300001200233490000700245520085700252856004201109       2019                        eng d00aIdentification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy0 aIdentification of ability to form biofilm in Candida parapsilosi  a509-5170 v143 a<p>Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials &amp; methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: The method showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.</p>  uhttps://doi.org/10.2217/fmb-2018-0297