01949nas a2200121   4500008004100000022001400041245011700055210006900172300001100241490000800252520149500260856007201755       2023                        eng d  a0304-389400aPhysiological and transcriptome profiling of Chlorella sorokiniana: A study on azo dye wastewater decolorization0 aPhysiological and transcriptome profiling of Chlorella sorokinia  a1324500 v4603 a<p>Over decades, synthetic dyes have become increasingly dominated by azo dyes posing a significant environmental risk due to their toxicity. Microalgae-based systems may offer an alternative for treatment of azo dye effluents to conventional physical-chemical methods. Here, microalgae were tested to decolorize industrial azo dye wastewater (ADW). Chlorella sorokiniana showed the highest decolorization efficiency in a preliminary screening test. Subsequently, the optimization of the experimental design resulted in 70% decolorization in a photobioreactor. Tolerance of this strain was evidenced using multiple approaches (growth and chlorophyll content assays, scanning electron microscopy (SEM), and antioxidant level measurements). Raman microspectroscopy was employed for the quantification of ADW-specific compounds accumulated by the microalgal biomass. Finally, RNA-seq revealed the transcriptome profile of C. sorokiniana exposed to ADW for 72&nbsp;h. Activated DNA repair and primary metabolism provided sufficient energy for microalgal growth to overcome the adverse toxic conditions. Furthermore, several transporter genes, oxidoreductases-, and glycosyltransferases-encoding genes were upregulated to effectively sequestrate and detoxify the ADW. This work demonstrates the potential utilization of C. sorokiniana as a tolerant strain for industrial wastewater treatment, emphasizing the regulation of its molecular mechanisms to cope with unfavorable growth conditions.</p>  uhttps://www.sciencedirect.com/science/article/pii/S030438942301733801487nas a2200121   4500008004100000245014800041210006900189260000800258300001600266490000700282520101500289856006101304       2022                        eng d00aAbsolute frequencies of H13C14N hydrogen cyanide transitions in the 1.5-µm region with the saturated spectroscopy and a sub-kHz scanning laser0 aAbsolute frequencies of H13C14N hydrogen cyanide transitions in   cNov  a5704–57070 v473 a<p>The wide span and high density of lines in its rovibrational spectrum render hydrogen cyanide a useful spectroscopic media for referencing absolute frequencies of lasers in optical communication and dimensional metrology. We determined, for the first time to the best of our knowledge, the molecular transitions' center frequencies of the H13C14N isotope in the range from 1526 nm to 1566 nm with 1.3&amp;\#x00A0;×&amp;\#x00A0;10\textminus10 fractional uncertainty. We investigated the molecular transitions with a highly coherent and widely tunable scanning laser that was precisely referenced to a hydrogen maser through an optical frequency comb. We demonstrated an approach to stabilize the operational conditions needed to maintain the constantly low pressure of the hydrogen cyanide to carry out the saturated spectroscopy with the third-harmonic synchronous demodulation. We demonstrated approximately a forty-fold improvement in the line centers' resolution compared to the previous result.</p>  uhttps://opg.optica.org/ol/abstract.cfm?URI=ol-47-21-5704